mouse a20 b cell lymphoma cells  (ATCC)

Journal: Cancer cell

Article Title: Lymphoma accelerates T cell and tissue aging

doi: 10.1016/j.ccell.2025.07.023

Figure Lengend Snippet: (A) Intracellular iron measured by BioTracker Fe 2+ in CD4 + and CD8 + T cells ( n = 6–14). (B) Intracellular iron measured by BioTracker Fe 2+ in CD44/CD62L CD4 + T cells memory populations ( n = 6–11). (C) Intracellular iron measured by BioTracker Fe 2+ in CD4 + T cells from OT-II mice ( n = 6). (D) Intracellular iron measured by BioTracker Fe 2+ in human CD4 + and CD8 + T cells from young and aged healthy donors or from R/R LBCL patients ( n = 5–6). (E) Percent DAPI − viable cells of YC or AC CD4 + and CD8 + T cells unstimulated or activated overnight ±10 mM ferric ammonium citrate (FAC) ( n = 3). (F) Aifm2 and Gpx4 gene expression in young and aged T cells activated overnight ( n = 3–4). (G) Percent DAPI − Annexin V − viable cells from human CD4 + (left) and CD8 + (right) T cells unstimulated or activated for 5 days ±10 mM FAC ( n = 3). (H) Percent DAPI − viable cells of YC or YL CD4 + and CD8 + T cells unstimulated or activated overnight ±10 mM FAC ( n = 3). (I) Aifm2 and Gpx4 gene expression in young T cells from control and lymphoma-bearing mice activated overnight ( n = 4). (J) ATAC-seq track around the Aifm2 locus and the accessibility index for the highlighted regions. (K) Experimental design for the A20 lymphoma BALB/cJ model. (L) Intracellular iron measured by BioTracker Fe 2+ in CD4 + and CD8 + T cells from control and A20 lymphoma-bearing BALB/cJ mice ( n = 4–8). (M) Total splenocyte number in control and A20 lymphoma-bearing mice ( n = 4–8). (N) Total splenic CD4 + and CD8 + T cells from control and A20 lymphoma-bearing mice ( n = 4–8). YC, YL, AC, AL are defined in . Data are mean ± SEM (A–D, L–N) or are mean ± SD (E–I). Data (A–D, K–N) are compiled from at least two independent experiments (2–6) and each solid dot represents a biological replicate; or data (E–I) were repeated at least twice with similar result and each hollow dot represents a technical replicate. Data were analyzed by a Student’s t test (A, C, D, F, I, and L–N). For (B, E, G, and H), data were analyzed by a two-way ANOVA with a Tukey’s test or Sidak’s test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001; n.s. = not significant. See also .

Article Snippet: A20 B-lymphoma cells (ATCC) were cultured in vitro in complete mouse media supplemented to 20% FBS and passaged every 3-4 days.

Techniques: Gene Expression, Control